9 Mar 2021 808 nm) and can analyze cells in 21 unique fluorescent color channels. 6- parameter analysis on an ACCURI C6 Flow Cytometer Analyzer

For more information about the BD Accuri C6 Plus, see the Appendix at the The optional Selectable Laser Module allows two or four fluorescence channels to

All thresholds are set on the Height signal for any given parameter. result is that each BD Accuri C6 cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. Data is digitally collected over a wide dynamic range of 7.2 decades (16 million channels of digital data), making all data available to users as needed. Gating strategies and BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research.

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Use the THRESHOLD button on the workspace. Threshold settings can be changed before, during, or after data acquisition. All thresholds are set on the Height signal for any given parameter. result is that each BD Accuri C6 cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages.

The BD Accuri C6 Plus personal flow cytometer, together with BD reagents, delivers something that every researcher should be afforded: the power of simple and personal flow cytometry. Whether you are sailing through the turbulent waters of Antarctica or working in a hectic lab, you can run your samples anytime, anywhere.

The event rate is close to 4000/s, and do not depend on the flow rate. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. With the broad dynamic range of the BD Accuri C6, it is often useful to focus in on a particular subset of channels or events.

Our lab got C6 5 years ago and updated to C6 plus recently. It is very easy to handle even though it only carry 4 channels.

If not, empty the waste into the white plastic sink.

You can check it from the website or catalog of the Accuri C6. In my opinion being an older one, it may not work. Cite. Article Snippet: 10 000–20 000 events were recorded in channel FL-4 and data assessed on BD Accuri C6 (BD Biosciences) flow cytometer. Techniques: Generated, Binding Assay, Western Blot, Modification, Expressing, Flow Cytometry, Cytometry, Software, Mutagenesis The C6 Flow Cytometer from Accuri Cytometers, Inc., is equipped with 2 lasers and 4 filters. Together with forward and side scatter, it can perform multiple color analysis in a single cytometry assay, which makes it feasible to perform cell counting and multiplexing, etc. At my lab, we have an Accuri C6 cytometer. We had a problem with its valves and it was solved in last November.
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images from all channels were acquired with an Using an Accuri C6 cytometer (BD Biosciences), 1 the spectral imaging detector, up to 32 channels can. 4 Sep 2018 Cells were analyzed by flow cytometry (Accuri C6). (B) Co-localization between FITC and TRITC channels was determined in the scatterplot fluorescence compensation, Accuri suggests nm) on the Accuri C6 Flow Cytometer®.

Analyzer: BD Accuri C6. Easy to use 2 LASER instrument: 488 nm, 640 nm; 4 fluorescence channels.
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RORTE 2013 presentation - "The BD Accuri C6 Flow Cytometer"-David Lee, BD Biosciences

The maximum recommended sample volume in a 12 x 75-mm tube is 2 mL. Never acquire more than 750 μ L from a single tube using the Medium fluidics setting, or 1,500 μ L using the Fast After 30 minutes fluorescence was measured using an Accuri C6 flow cytometer ( BD Biosciences, Oxford, UK ). Data represent mean A 450 ± SE of three separate donors. ** P . Article Snippet: After 30 minutes fluorescence was measured using an Accuri C6 flow cytometer (BD Biosciences, Oxford, UK). BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed.